Symptoms of a twolined chestnut borer infestation are first seen in mid-July. The first branches to be attacked are usually at the top of the crown or fully exposed to sunlight. Leaves on infested branches die, turn orange, and remain on the branches for weeks or months. The persistence of dead leaves is one characteristic that distinguishes twolined chestnut borer attack from oak wilt infection. After a year of infestation, the top third of the crown will be dead and leafless, and the leaves on the middle third or bottom two-thirds will die and persist in the canopy for several weeks or months. In its second summer of infestation, the canopy often has three layers—dead, red, and green.
Larvae of twolined chestnut borer can be found in dead and dying branches, where they make winding feeding tunnels. Adult beetles chew out of the bark, leaving D-shaped exit holes. Images of the insect's life stages can be seen at the University of Minnesota Extension bronze birch borer and twolined chestnut borer webpage.
Oaks that die from oak wilt and Armillaria root disease are susceptible to secondary attack by twolined chestnut borer; the presence of exit holes and galleries does not necessarily mean that twolined chestnut borer killed the tree.