Treefrogs are considerably smaller than toads or true frogs. All treefrogs have toe pads, though they vary in size among the species. Species with large toe pads can climb high into trees to feed.
During the breeding season, males call from perches on vegetation in or near standing water. Female treefrogs lay eggs singly or in small clusters, attaching them to aquatic vegetation.
Many species of treefrogs overwinter in leaf litter or under rocks or logs. Some are capable of withstanding freezing temperatures by creating their own antifreeze. Their liver converts glycogen into glucose (glycerol in gray treefrogs) which is pumped into the frog's organs preventing ice formation. Portions of their body cavity can tolerate the formation of ice crystals.